离子色谱法同时测定胞外培养液中的有机酸与核苷酸

建立了一种利用阴离子交换色谱柱分离,抑制型电导检测器同时测定乳酸、丙酸、丙酮酸、苹果酸、磷酸二羟丙酮、柠檬酸、异柠檬酸7种有机酸和单磷酸核苷酸(AMP)、双磷酸核苷酸(ADP)、三磷酸核苷酸(ATP)3种核苷酸的方法。采用IonPacAS11-HC阴离子交换柱,KOH梯度洗脱,流速1.00mL/min,柱温30℃,进样体积25μL。方法的相对标准偏差为0.39%～4.69%,线性相关系数为0.9906～0.9996,加标回收率为80.71%～94.02%,方法可用于嗜热厌氧菌胞外培养液中有机酸和核苷酸的同时测定。     

羟基系列卟啉对三聚氰胺的检测研究

研究了四苯基卟啉(TPP)及5种羟基系列卟啉对三聚氰胺的检测,结果表明,TPP及羟基系列卟啉可以用来检测三聚氰胺,三聚氰胺与卟啉发生作用,导致其soret带特征峰强度的变化,其中邻二羟基卟啉对三聚氰胺的检测效果最好,检测限为0.2μg/L,摩尔吸光系数ε为0.6×106L·mol-1.cm-1。并对其作用机理进行了简要分析。     

基于新型可视化学传感阵列的氨基酸快速识别

以卟啉和其衍生物及指示剂为传感元件,构建了一种对氨基酸敏感的可视传感阵列.可视化学传感阵列以交互响应的敏感元件组成阵列,对不同物质产生特异的响应,并通过信号识别处理系统,将检测结果以图谱的方式显示,实现检测的可视化.研究中筛选了对氨基酸敏感的36种化学物质,构建了6×6的传感阵列,使用自主研发的阵列数据采集与处理系统,对10种具有代表性的常见氨基酸进行了检测,氨基酸溶液与阵列的反应时间为5 min.对实验检测结果数据采用主成分分析和判别分析进行了计算和分析.实验结果显示,通过阵列响应的可视差图可以将浓度为375 μmol/L的10种氨基酸明显区分.判别分析结果显示,本可视阵列对氨基酸识别的准确率达到97%.二维主成分散点图和判别分析散点图对10种氨基酸都有显著分辨效果.本可视传感阵列可用于氨基酸的快速识别.     

Novel fluorescent security marker. Part I: morphological and optical properties of 2-amino-6-ethoxy-4-[4-(4-morpholinyl)phenyl]-3,5-pyridinedicarbonitrile nanoparticles

Newly synthesized fluorescent nanoparticles of 2-amino-6-ethoxy-4-[4-(4-morpholinyl)phenyl]-3,5-pyridinedicarbonitrile have been developed and characterized for possible applications as security marker in paper documents. Nanoparticles have been prepared by reprecipitation in water under sonication. The size and the shape of these nanoparticles, characterized by light scattering and atomic force microscopy, have been found to be highly dependent on sonication power. Typical sizes range from tens to hundreds of nanometers. Furthermore, a remarkable increase in the fluorescence yield has been observed as nanoparticles sizes decrease. Finally, all of the above features, together with the striking stability of optical and mechanical properties over the course of months, allow for straightforward applications that rely on strong and stable fluorescence such as marking important or valuable documents.     

用同步辐射X射线衍射技术分析GaN/Si外延膜的结构与应变

选用有AlN和AlGaN缓冲层的GaN/Si作为测试样品,采用同步辐射X射线衍射(SRXRD)技术对样品外延膜(GaN)的几何结构、晶格常量及其应变进行了分析.结果表明,同步辐射X射线衍射实验可以作为一种有效的技术手段,测试固体结构及应变.     

Copper(II) complexes of 4-formylantipyrine N(4)-substitutedthiosemicarbazones

Copper(II) complexes between three 4-formylantipyrine N(4)-substituted thiosemicarbazones and four copper(II) salts have been prepared and characterized. I.r., electronic and e.s.r. spectra of the complexes, as well as i.r., electronic and 1H and 13C-n.m.r. spectra of the thiosemicarbazones, have been obtained. Both mononuclear and binuclear complexes have been formed with bridging ligands for the latter, being either chloro or acetato. The stoichiometries of the complexes are dependent on both the steric requirements of the thiosemicarbazone ligands and the electronic effects of their N(4)-substituents.     

用纵横波声时比法测螺栓轴力精度研究

用纵横波测量螺栓轴力的技术已取得较大发展,本文介绍了纵横波比值法原理,通过理论推导,获得比值法计算的简易公式。建立试验平台,用现场用螺栓进行标定和测量,得出比值法在工程实践状态下的测量误差为10%以内,基本能满足工程应用的要求。此外,针对试验结果,本文还初步探讨了误差出现的原因以及提高精度的方法。本文介绍的比值法,无需精确测量标定螺栓长度和纵横波声速,现场应用更加简洁,具有较大的工程应用价值。     

Synthesis and characterization of iron(III), manganese(II), cobalt(II), nickel(II), copper(II) and zinc(II) complexes of salicylidene-N-anilinoacetohydrazone (H2L1) and 2-hydroxy-1-naphthylidene-N-anilinoacetohydrazone (H2L2)

Salicylidene-N-anilinoacetohydrazone (H(2)L(1)) and 2-hydroxy-1-naphthylidene-N-anilinoacetohydrazone (H(2)L(2)) and their iron(III), manganese(II), cobalt(II), nickel(II), copper(II) and zinc(II) complexes have been synthesized and characterized by IR, electronic spectra, molar conductivities, magnetic susceptibilities and ESR. Mononuclear complexes are formed with molar ratios of 1:1, 1:2 and 1:3 (M:L). The IR studies reveal various modes of chelation. The electronic absorption spectra and magnetic susceptibility measurements show that the iron(III), nickel(II) and cobalt(II) complexes of H(2)L(1) have octahedral geometry. While the cobalt(II) complexes of H(2)L(2) were separated as tetrahedral structure. The copper(II) complexes have square planar stereochemistry. The ESR parameters of the copper(II) complexes at room temperature were calculated. The g values for copper(II) complexes proved that the Cu-O and Cu-N bonds are of high covalency.     

一种可视化液体传感阵列农药检测新方法

以筛选出的7种染料与合成的纳米金共同构建了液体传感器阵列,每个敏感点对不同农药样品产生不同的响应光谱,通过酶标仪采集光谱数据,结合主成分分析(PCA)、分层聚类分析(HCA)、判别分析(LDA)等模式识别方法建立了一种快速检测有机磷、氨基甲酸酯、有机氯、拟除虫菊酯类农药残留的新方法。PCA结果表明,前三个主成分反映了总信息量的92.69%,且能够对5种农药进行区分;HCA结果表明,对25个样品能够正确的归类;LDA结果表明,对5种农药识别的准确率达100%。表明这种可视化的液体阵列可为农药残留检测提供一个可靠平台,在农药残留检测中具有潜在的应用价值。     

单质碘掺杂纳米TiO2光催化剂的制备及性能

以钛酸四异丙酯和单质碘为起始原料,用改进的溶胶-凝胶法制备了掺碘的纳米TiO2的前驱体,经500 ℃煅烧得到棕黄色TiO2/I2纳米粉末.采用X射线衍射(XRD),紫外-可见(UV-Vis)漫反射光谱,透射电镜(TEM)等手段对TiO2/I2粉末进行了表征,并研究了其光催化性能.结果表明,掺杂的碘存在于TiO2内部纳米孔洞中,不会因为高温而被氧化;光催化降解甲基橙反应是一级反应;TiO2掺杂碘单质后对光响应波长拓展至可见光范围,对甲基橙的降解效率可提高15%以上.